The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) at Faculty of Health & Medical Science at the University of Copenhagen is looking for a research assistant to join the Brickman group starting September 1st or upon agreement.

The position will explore enhancer regulation in response to FGF/ERK signaling as a means to understand how transcription factors interface with signaling to regulate cell fate choice in early development as part of an ERC advanced grant program, “Signaling decoded in ENhanCEr states – a molecular basis for plasticity in development and differentiation (SENCE).” These positions can also be associated with a second project focused on a similar phenomena in Ventral Foregut derived from human pluripotent stem cells, where we have observed proliferation leads to the commissioning of appropriate enhancers and decommissioning of inappropriate ones.

Our research
The Brickman group is interested in the transcriptional basis for cell fate choice, exploring both fundamental mechanisms of transcriptional regulation by signaling and how gene regulatory networks define cell potency and fate in development and differentiation. We focus principally on pluripotent stem cells and their differentiation to various types of endoderm as a model system, both embryonic and extra-embryonic. Our recent work points to new paradigms for how transcription factors regulate enhancer potential and interpret signaling, including the finding that response to MAP kinase (ERK) signaling, plasticity and regeneration in early development is dependent on transcription factor occupancy. This project will explore how enhancers are activated in response to ERK signaling, understanding the molecular basis for enhancer activity by exploiting signaling models and understanding how enhancer status safeguards early developmental potential in a number of different model systems.

Job description
We are looking for positions that will focus on different aspects enhancer function and regulation, the interface with signaling and how they are influenced by the cell cycle.
As a research assistant in the Brickman lab, you will be working in a field of stem cell and molecular biology. You will be part of an international interdisciplinary team of scientists focusing on understanding enhancer regulation, gene regulatory networks and exploiting these networks to develop new technologies for cell type specification in vitro.
We work with in vivo and in vitro systems in both mouse and human. For this project, you will work with either mouse embryos, mouse and human embryonic stem cell (ESCs), human Ventral Foregut stem cells, human hypoblast stem cells and three-dimensional synthetic models for human and mouse development.

Select Recent References
Linneberg-Agerholm M, Sell AC, Redo-Riveiro A, Proks M, Knudsen TE, Perera M, and Brickman JM. Joshua M. Brickman. Enhancer status in the primitive endoderm supports unrestricted lineage plasticity in regulative development. bioRxiv 2023.05.20.540779; doi: https://doi.org/10.1101/2023.05.20.540779

Redó-Riveiro A, Al-Mousawi J, Linneberg-Agerholm M, Proks M, Perera M, Salehin N, Brickman JM. Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation. Stem Cell Reports. 2024 Feb 13;19(2):174-186. doi: 10.1016/j.stemcr.2023.12.002.

Wenger A, Biran A, Alcaraz N, Redó-Riveiro A, Sell AC, Krautz R, Flury V, Reverón-Gómez N, Solis-Mezarino V, Völker-Albert M, Imhof A, Andersson R, Brickman JM, Groth A. Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity. Nat Genet. 2023

Sep;55(9):1567-1578. doi: 10.1038/s41588-023-01476-x.

Knudsen TE, Hamilton WB, Proks M, Lykkegaard M, Linneberg-Agerholm M, Nielsen AV, Perera M, Malzard LL, Trusina A, Brickman JM. A bipartite function of ESRRB can integrate signaling over time to balance self-renewal and differentiation. Cell Syst. 2023 Sep 20;14(9):788-805.e8. doi: 10.1016/j.cels.2023.07.008.

Wong YF, Kumar Y, Proks M, Herrera JAR, Rothová MM, Monteiro RS, Pozzi S, Jennings RE, Hanley NA, Bickmore WA, Brickman JM. Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation. Nat Cell Biol. 2022 Mar;25(3):481-492. doi: 10.1038/s41556-022-01075-8.

Knudsen, T. E. and Brickman, J. M. (2020). Can a Cell Put Its Arms around a Memory? Cell Stem Cell 26, 609–610.

Hamilton, W.B., Mosesson, Y., Monteiro, R.S., Emdal, K.B., Knudsen, T.E., Francavilla, C., Barkai, N., Olsen, J.V., and Brickman, J.M. (2019). Dynamic Lineage Priming is Driven via Direct Enhancer Regulation by ERK. Nature, 575, 355–360, doi:10.1038/s41586-019-1732-z.

Responsibilities

• To implement state of the art genomic and proteomic technologies to understand the basis of enhancer activity in early development and differentiation.
• To develop new approaches to identify enhancer specific co-factors involved in signaling response.
• To define regulatory networks and signaling response.
• To optimize new molecular and biochemical approaches to single locus and single molecule functional assays.

Qualifications

• An equivalent of a master’s degree in Developmental, Stem Cell or Molecular Biology and/or Bioinformatics.
• Experience with computational analysis of next generation sequencing data.
• Experience with embryonic stem cell culture.
• Molecular biology, western blots, RT-PCR etc.
• English communication skills, both oral and written are a must.

We offer

• A stimulating research environment and collaborative work with physicists from Niels Bohr Institute and hospital.
• Usage of state-of-the-art technologies for stem cell research.
• Possibilities for continued education and training.

Place of employment
The place of employment is at Novo Nordisk Foundation Center for Stem Cell Medicine, reNEW, University of Copenhagen. We offer creative and stimulating working conditions in dynamic and international research environment.

Terms of salary, work, and employment
The average weekly working hours are 37 hours per week.

The position is a fixed-term position limited to a period of one year. The starting date is 1 September 2024 or after agreement.

Salary, pension and other conditions of employment are set in accordance with the Agreement between the Ministry of Taxation and AC (Danish Confederation of Professional Associations) or other relevant organisation. The employer will pay an additional 17.1 % to your pension fund.

The position is covered by the Job Structure for Academic Staff at Universities 2020.

We encourage all - regardless of their personal background and status - to apply.

The application must include

1. Motivated letter of application (max. one page)
2. Curriculum vitae incl. education, experience, previous employments, programming languages and other relevant skills
3. List of references with contact information
4. Publication list (if possible)
5. Certified copy of original Master of Science diploma and transcript of records in the original language, including an authorized English translation if issued in other language than English or Danish. If not completed, a certified/signed copy of a recent transcript of records or a written statement from the institution or supervisor is accepted. As a prerequisite for a research assistant employment, your master’s degree must be equivalent to a Danish master’s degree. We encourage you to read more in the assessment database: https://ufm.dk/en/education/recognition-and-transparency/find-assessments/assessment-database. Please note that we might ask you to obtain an assessment of your education performed by the Ministry of Higher Education and Science

Application deadline: 1 July 2024, 23.59pm CET

We reserve the right not to consider material received after the deadline, and not to consider applications that do not live up to the abovementioned requirements.

Questions
For further information contact Professor Joshua Brickman [email protected]

The further process
After the expiry of the deadline for applications, the authorized recruitment manager selects applicants for assessment on the advice of the hiring committee. All applicants are then immediately notified whether their application has been passed for assessment by an unbiased assessor.

The assessor makes a non-prioritized assessment of the academic qualifications and experience with respect to the above-mentioned area of research, techniques, skills, and other requirements listed in the advertisement.

Once the assessment work has been completed each applicant has the opportunity to comment on the part of the assessment that relates to the applicant him/herself.

You find information about the recruitment process at: http://employment.ku.dk/faculty/recruitment-process/

The applicants will be assessed according to the Ministerial Order no. 242 of 13 March 2012 on the Appointment of Academic Staff at Universities.

The University of Copenhagen wish to reflect the diversity of society and encourage all qualified candidates to apply regardless of personal background.

Kilde: Jobnet.dk